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Genoptix Inc automated quantitative analysis (aqua)
Automated Quantitative Analysis (Aqua), supplied by Genoptix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+quantitative+analysis+(aqua/aqua++automated+quantitative+analysis++method/pm40521789-359-26-28
Average 90 stars, based on 1 article reviews
automated quantitative analysis (aqua) - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Expressing:

Article Title: Deep exploration of the immune infiltrate and outcome prediction in testicular cancer by quantitative multiplexed immunohistochemistry and gene expression profiling
Article Snippet: We performed multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix Inc)(19) to assess PD-1, PD-L1, CD3, CD4, CD8, CD25, and FOXP3.

Article Title: Deep exploration of the immune infiltrate and outcome prediction in testicular cancer by quantitative multiplexed immunohistochemistry and gene expression profiling
Article Snippet: We performed multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix, Inc.) 19 to assess PD-1, PD-L1, CD3, CD4, CD8, CD25, and FOXP3.

Article Title: Immunotherapy in Testicular Germ Cell Tumors
Article Snippet: ( ) , - Increased PD-L1 expression and elevated PD-1/PD-L1 spatial interaction predominantly found in seminomas and its correlation with good prognosis disease , Multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix, Inc.) Targeted gene expression profiling (Nanostring nCounter Immune panel).

Fluorescence:

Article Title: Deep exploration of the immune infiltrate and outcome prediction in testicular cancer by quantitative multiplexed immunohistochemistry and gene expression profiling
Article Snippet: We performed multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix Inc)(19) to assess PD-1, PD-L1, CD3, CD4, CD8, CD25, and FOXP3.

Article Title: Deep exploration of the immune infiltrate and outcome prediction in testicular cancer by quantitative multiplexed immunohistochemistry and gene expression profiling
Article Snippet: We performed multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix, Inc.) 19 to assess PD-1, PD-L1, CD3, CD4, CD8, CD25, and FOXP3.

Article Title: Immunotherapy in Testicular Germ Cell Tumors
Article Snippet: ( ) , - Increased PD-L1 expression and elevated PD-1/PD-L1 spatial interaction predominantly found in seminomas and its correlation with good prognosis disease , Multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix, Inc.) Targeted gene expression profiling (Nanostring nCounter Immune panel).

Immunohistochemistry:

Article Title: Deep exploration of the immune infiltrate and outcome prediction in testicular cancer by quantitative multiplexed immunohistochemistry and gene expression profiling
Article Snippet: We performed multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix Inc)(19) to assess PD-1, PD-L1, CD3, CD4, CD8, CD25, and FOXP3.

Article Title: Deep exploration of the immune infiltrate and outcome prediction in testicular cancer by quantitative multiplexed immunohistochemistry and gene expression profiling
Article Snippet: We performed multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix, Inc.) 19 to assess PD-1, PD-L1, CD3, CD4, CD8, CD25, and FOXP3.

Article Title: Immunotherapy in Testicular Germ Cell Tumors
Article Snippet: ( ) , - Increased PD-L1 expression and elevated PD-1/PD-L1 spatial interaction predominantly found in seminomas and its correlation with good prognosis disease , Multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix, Inc.) Targeted gene expression profiling (Nanostring nCounter Immune panel).

Targeted Gene Expression:

Article Title: Deep exploration of the immune infiltrate and outcome prediction in testicular cancer by quantitative multiplexed immunohistochemistry and gene expression profiling
Article Snippet: We performed multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix Inc)(19) to assess PD-1, PD-L1, CD3, CD4, CD8, CD25, and FOXP3.

Article Title: Deep exploration of the immune infiltrate and outcome prediction in testicular cancer by quantitative multiplexed immunohistochemistry and gene expression profiling
Article Snippet: We performed multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix, Inc.) 19 to assess PD-1, PD-L1, CD3, CD4, CD8, CD25, and FOXP3.

Article Title: Immunotherapy in Testicular Germ Cell Tumors
Article Snippet: ( ) , - Increased PD-L1 expression and elevated PD-1/PD-L1 spatial interaction predominantly found in seminomas and its correlation with good prognosis disease , Multiplexed fluorescence immunohistochemistry (FIHC) combined with automated quantitative analysis (AQUA; Genoptix, Inc.) Targeted gene expression profiling (Nanostring nCounter Immune panel).



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Validation <t>of</t> <t>EGFR:GRB2</t> PLA in NSCLC cell lines. (A) <t>AQUA®</t> images of FFPE cell lines with different EGFR expression levels and NSCLC FFPE cell lines with different EGFR mutation status. EGFR:GRB2 PLA (red), cytokeratin 42, DAPI (4′,6-diamidino-2-phenylindole) (blue). (B) Western blots of phosphorylated Tyr1068 EGFR, total EGFR, and GRB2. β-tubulin was used as a loading control. Blots are representative of two independent experiments. (C) Technical control of EGFR:GRB2 PLA. AQUA® images of EGFR mutant patient case with complete PLA reaction and without EGFR primary antibody. EGFR:GRB2 PLA (red), cytokeratin 42, DAPI (4′,6-diamidino-2-phenylindole) (blue). Images in (A) and (C) are representative of two independent experiments (D) Reproducibility and core-to-core variability of EGFR:GRB2 quantified by AQUA®.
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Image Search Results


Validation of EGFR:GRB2 PLA in NSCLC cell lines. (A) AQUA® images of FFPE cell lines with different EGFR expression levels and NSCLC FFPE cell lines with different EGFR mutation status. EGFR:GRB2 PLA (red), cytokeratin 42, DAPI (4′,6-diamidino-2-phenylindole) (blue). (B) Western blots of phosphorylated Tyr1068 EGFR, total EGFR, and GRB2. β-tubulin was used as a loading control. Blots are representative of two independent experiments. (C) Technical control of EGFR:GRB2 PLA. AQUA® images of EGFR mutant patient case with complete PLA reaction and without EGFR primary antibody. EGFR:GRB2 PLA (red), cytokeratin 42, DAPI (4′,6-diamidino-2-phenylindole) (blue). Images in (A) and (C) are representative of two independent experiments (D) Reproducibility and core-to-core variability of EGFR:GRB2 quantified by AQUA®.

Journal: Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

Article Title: EGFR:GRB2 protein co-localization is a prognostic factor unrelated to overall EGFR expression or EGFR Mutation in Lung Adenocarcinoma

doi: 10.1016/j.jtho.2016.06.025

Figure Lengend Snippet: Validation of EGFR:GRB2 PLA in NSCLC cell lines. (A) AQUA® images of FFPE cell lines with different EGFR expression levels and NSCLC FFPE cell lines with different EGFR mutation status. EGFR:GRB2 PLA (red), cytokeratin 42, DAPI (4′,6-diamidino-2-phenylindole) (blue). (B) Western blots of phosphorylated Tyr1068 EGFR, total EGFR, and GRB2. β-tubulin was used as a loading control. Blots are representative of two independent experiments. (C) Technical control of EGFR:GRB2 PLA. AQUA® images of EGFR mutant patient case with complete PLA reaction and without EGFR primary antibody. EGFR:GRB2 PLA (red), cytokeratin 42, DAPI (4′,6-diamidino-2-phenylindole) (blue). Images in (A) and (C) are representative of two independent experiments (D) Reproducibility and core-to-core variability of EGFR:GRB2 quantified by AQUA®.

Article Snippet: Quantitative measurements of EGFR:GRB2 PLA and EGFR immunofluorescence (IF) were performed using AQUA method (Automated Quantitative Analysis) (Genoptix Medical Laboratory), quantifying fluorescent signal within subcellular compartments, as described previously 18 .

Techniques: Biomarker Discovery, Expressing, Mutagenesis, Western Blot, Control